LOCUS       sequence-115241-        2367 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mCherry fused with out-of-frame blasticidin-S resistance 
            gene with a linker containing a target for TALEN/CRISPR. Can use as 
            a surrogate target to select cells expressing active TALEN/CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Niccheri F, Pecori R, Conticello SG
  TITLE     An efficient method to enrich for knock-out and knock-in cellular 
            clones using the CRISPR/Cas9 system.
  JOURNAL   Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
  PUBMED    28421278
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y."
COMMENT     Sequence Label: pBSR (mCherry-surrogateTarget-bsr)
FEATURES             Location/Qualifiers
     source          1..2367
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..722
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(160..178)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     428..447
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          1564..1629
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1759..1779
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2204..2285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2241..2260)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2318..2334)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2318..2334)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctagcggatt caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
       61 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      181 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      241 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      301 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      361 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      481 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      541 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      601 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      661 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      721 agaagcttcg tctctgtcgg ataatctgca actccagtct ttccagaaga tgggcgggag
      781 tcttctgggc aaagatctgc gagacggaat tctgctggtt attgtgctgt ctcatcattt
      841 tggcaaagaa ttaattcaag cttggattca tttttgcaga aatcggagga agaagaatat
      901 atgaaaacat ttaacatttc tcaacaagat ctagaattag tagaagtagc gacagagaag
      961 attacaatgc tttatgagga taataaacat catgtgggag cggcaattcg tacgaaaaca
     1021 ggagaaatca tttcggcagt acatattgaa gcgtatatag gacgagtaac tgtttgtgca
     1081 gaagccattg cgattggtag tgcagtttcg aatggacaaa aggattttga cacgattgta
     1141 gctgttagac acccttattc tgacgaagta gatagaagta ttcgagtggt aagtccttgt
     1201 ggtatgtgta gggagttgat ttcagactat gcaccagatt gttttgtgtt aatagaaatg
     1261 aatggcaagt tagtcaaaac tacgattgaa gaactcattc cactcaaata tacccgaaat
     1321 taaaagtttt accataccaa gcttggctgc tgcctgaggc tggacgacct cgcggagttc
     1381 taccggcagt gcaaatccgt cggcatccag gaaaccagca gcggctatcc gcgcatccat
     1441 gcccccgaac tgcaggagtg gggaggcacg atggccgctt tggtccggat ctttgtgaag
     1501 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     1561 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     1621 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     1681 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     1741 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     1801 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     1861 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     1921 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     1981 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     2041 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     2101 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2161 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     2221 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2281 actgcattct agttgtggga attcctgcag cccgggggat ccactagttc tagagcggcc
     2341 gccaccgcgg tggagctcca gcttttg
//
