LOCUS       sequence-115242-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the wild type of Staphylococcus aureus sortase A in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtAwoH
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(518..562)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     RBS             572..594
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        633..688
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    634..652
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    664..682
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 cagacaaaaa aaatgtcgca caatgtgcgc catttttcac ttcacaggtc gtttaaactt
       61 atttgacttc tgtagctaca aagattttac gtttttccca aacgcctgtc ttttcattgt
      121 aatcatcaca agtaattaat gttaattgtt tatctttacc tttttgttca tctagaactt
      181 ctacatctgt aggcttaaca tctcttatac ttgtcatttt atacttacgt gtttcattac
      241 caactttaaa gtacaccata ctaccttttt tggctgcttt aagatttgta aattgatagt
      301 tcggacggtc aatgaaagtg tgtcctgcaa ttgaaatatt ttgatcatct agtgattcgt
      361 tttcttctgc aaagcttaca cctctattta attgttcagg tgttgctggt cctggatata
      421 ctggttcttt aatatcagca tctggaattt caatatagcc tgccactttc gatttatctt
      481 tcggaatttg aggtttagct tgggtaccca gatctgggct gtccatgtgc tggcgttcga
      541 atttagcagc agcggtttct ttcatggtat atctccttct taaagttaaa caaaattatt
      601 tctagatttt tgtcgaacta ttcatttcac ttttctctat cactgatagg gagtggtaaa
      661 ataactctat caatgataga gtgtcaacaa aaattaggaa ttaatcatct ggccattcga
      721 tggtgtcggg tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggcagaa
      781 ttacaactta tatcgtatgg ggctgac
//
