LOCUS       sequence-115243-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, for construction of B-galactosidase protein 
            fusions, frame 2. See Depositor Comments below for information 
            regarding the lacZ sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Gm-LacZ20
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     155..177
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(260..307)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(348..353,354..370,371..376)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gcttctggtg ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg caactgttgg
       61 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
      121 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
      181 ggagcaagct tggctgcagg tcgacggatc cccgggaatt cgagctcatg catgatcgaa
      241 ttagcttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
      301 gaacttcaag atcccctgat tccctttgtc aacagcaatg gatcgaattg acataagcct
      361 gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
      421 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
      481 tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
      541 gtttgatgtt atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc
      601 agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc
      661 ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga
      721 gacgtagcca cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt
      781 agtaagacat tcatcgcgct tgctgccttc ga
//
