LOCUS       sequence-115244-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, for construction of B-galactosidase protein 
            fusions, frame 2. See Depositor Comments below for information 
            regarding the lacZ sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Gm-LacZ20
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      48..82
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      195..281
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    327..374
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
       61 gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg agtggcaggg
      121 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctgggg taatgactct
      181 ctagcttgag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      241 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgcta ggagcttgcg
      301 gcccggacga tcgaattggg gatcttgaag tacctattcc gaagttccta ttctctagaa
      361 agtataggaa cttcagagcg cttttgaagc tgatgtgctt aaaaacttac tcaatggaat
      421 tctagatcga attggccgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag
      481 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
      541 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
      601 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg
      661 agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag
      721 atctcactac gcggctgctc
//
