LOCUS       sequence-115245-         220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRT cassette base vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFRT2
FEATURES             Location/Qualifiers
     source          1..220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..68
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    161..208
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gagctcgaat tggggatctt gaagtaccta ttccgaagtt cctattctct agaaagtata
       61 ggaacttcag agcgcttttg aagctgatgt gcttaaaaac ttactcaatg gaataattct
      121 agatatccat tgctgttgac aaagggaatc aggggatctt gaagttccta ttccgaagtt
      181 cctattctct agaaagtata ggaacttcag agcgcttttg
//
