LOCUS       sequence-115246-         310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRT cassette base vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFRT2
FEATURES             Location/Qualifiers
     source          1..310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(55..102)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(195..242)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tcgactctag aggatccccg ggtaccgagc tcgaattagc ttcaaaagcg ctctgaagtt
       61 cctatacttt ctagagaata ggaacttcgg aataggaact tcaagatccc ctgattccct
      121 ttgtcaacag caatggatat ctagaattat tccattgagt aagtttttaa gcacatcagc
      181 ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggtact
      241 tcaagatccc caattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
      301 caagcttggc
//
