LOCUS       sequence-115247-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 
            (eft-3) promoter and contains a C. briggsae unc-119 rescuing 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Katic I, Xu L, Ciosk R
  TITLE     CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of 
            Templates for Homology-Mediated Repair and Knock-Ins by 
            Homology-Independent DNA Repair.
  JOURNAL   G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 
            10.1534/g3.115.019273.
  PUBMED    26044730
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 
            10.1534/g3.115.019273."
COMMENT     Sequence Label: pIK137
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(444..468)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      473..796
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
ORIGIN
        1 atggcatgga tgaactatac aaatgcccgg gggatcggtg gagctccacc ggtggcggcc
       61 gctctagaac tagtccacca aagccatctg ccaagggagc caagaaggcc gccaagaccg
      121 tcgttgccaa gccaaaggac ggaaagaaga gacgtcatgc ccgcaaggaa tcgtactccg
      181 tctacatcta ccgtgttctc aagcaagttc acccagacac cggagtctcc tccaaggcca
      241 tgtctatcat gaactccttc gtcaacgatg tattcgaacg catcgcttcg gaagcttccc
      301 gtcttgctca ttacaacaaa cgctcaacga tctcatcccg cgaaattcaa accgctgtcc
      361 gtttgattct cccaggagaa cttgccaagc acgccgtgtc tgagggaacc aaggccgtca
      421 ccaagtacac ttccagcaag taaacccagc tttcttgtac aaagtgggat aaatgcaaaa
      481 tcctttcaag cattcccttc ttctctatca ctcttctttc tttttgtcaa aaaattctct
      541 cgctaattta tttgcttttt taatgttatt attttatgac tttttatagt cactgaaaag
      601 tttgcatctg agtgaagtga atgctatcaa aatgtgattc tgtctgatgt actttcacaa
      661 tctctcttca attccatttt gaagtgcttt aaacccgaaa ggttgagaaa aatgcgagcg
      721 ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa aagaggaaac tttattgtgc
      781 cgccaagaaa aaagtctcac aactatcgcc tagttctaga cattctctaa tgaaaaaatc
      841 tttcagttga aattgaaaat gagttaaagt tggagttttt attgaaaaca gatttccgtg
      901 tgattagtgt ttttagcgag tgtgacagga cagcgaaaaa atatagaaac aaggggggaa
      961 ctgaaaagct taggaatgca ttgaacatga gaaggggaag gggaaggaac aaactagaca
     1021 ggaattattg gaatttaatc
//
