LOCUS       sequence-115251-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of 
            Staphylococcus aureus sortase A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA7
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             28..50
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..107
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             114..158
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(793..812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atagttcgac aaaaatctag aaataatttt gtttaacttt aagaaggaga tataccatgg
       61 gccaccatca tcatcatcat tcttctggtc tggtgccacg cggttctggt atgaaagaaa
      121 ccgctgctgc taaattcgaa cgccagcaca tggacagccc agatctgggt acccaagcta
      181 aacctcaaat tccgaaagat aaatcgaaag tggcaggcta tattgaaatt ccagatgctg
      241 atattaaaga accagtatat ccaggaccag caacacggga acaattaaat agaggtgtaa
      301 gctttgcaaa ggaaaatgcc tcactagatg atcaaaatat ttcaattgca ggacacactt
      361 tcattgaccg tccgaactat caatttacaa atcttaaagc agccaaaaaa ggtagtatgg
      421 tgtactttaa agttggtaat gaaacacgta agtataaaat gacaagtata agaaacgtta
      481 agcctacagc cgtagaagtt ctagatgaac aaaaaggtaa agataaacaa ttaacattaa
      541 ttacttgtga tgattacaat gaagagacag gcgtttggga aacccgtaaa atctttgtag
      601 ctacagaagt caaataagtt taaacgacct gtgaagtgaa aaatggcgca cattgtgcga
      661 catttttttt gtctgccgtt taccgctact gcgtcacgga tctccacgcg ccctgtagcg
      721 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      781 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      841 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      901 tcgaccccaa aaaact
//
