LOCUS       sequence-115254-        4035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 
            / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4035)
  TITLE     Davidson Fluorescent Proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mEos3.2-Vinculin-N-21
FEATURES             Location/Qualifiers
     source          1..4035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3303..3983
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cgcaatgcta gtgaaccgtc agatccgcta gcaccgccac catgccagtg tttcatacgc
       61 gcacgatcga gagcatcctg gagccggtgg cacagcagat ctcccacctg gtgataatgc
      121 acgaggaggg cgaggtggac ggcaaagcca ttcctgacct caccgcgccc gtggccgccg
      181 tgcaggcggc cgtcagcaac ctcgtccggg ttggaaaaga gactgttcaa accactgagg
      241 atcagatttt gaagagagat atgccaccag catttattaa ggttgagaat gcttgcacca
      301 agcttgtcca ggcagctcag atgcttcagt cagaccctta ctcagtgcct gctcgagatt
      361 atctaattga tgggtcaagg ggcatcctct ctggaacatc agacctgctc cttaccttcg
      421 atgaggctga ggtccgtaaa attattagag tttgcaaagg aattttggaa tatcttacag
      481 tggcagaggt ggtggagact atggaagatt tggtcactta cacaaagaat cttgggccag
      541 gaatgactaa gatggccaag atgattgacg agagacagca ggagctcact caccaggagc
      601 accgagtgat gttggtgaac tcgatgaaca ccgtgaaaga gttgctgcca gttctcattt
      661 cagctatgaa gatttttgta acaactaaaa actcaaaaaa ccaaggcata gaggaagctt
      721 taaaaaatcg caattttact ctagaaaaaa tgagtgctga aattaatgag ataattcgtg
      781 tgttacaact cacctcttgg gatgaagatg cctgggccag caaggacact gaagccatga
      841 agagagcatt ggcctccata gactccaaac tgaaccaggc caaaggttgg ctccgtgacc
      901 ctagtgcctc cccaggggat gctggtgagc aggccatcag acagatctta gatgaagctg
      961 gaaaagttgg tgaactctgt gcaggcaaag aacgcaggga gattctggga acttgcaaaa
     1021 tgctagggca gatgactgat caagtggctg acctccgtgc cagaggacaa ggatcctcac
     1081 cggtggccat gcagaaagct cagcaggtat ctcagggtct ggatgtgctc acagcaaaag
     1141 tggaaaatgc agctcgcaag ctggaagcca tgaccaactc aaagcagagc attgcaaaga
     1201 agatcgatgc tgctcagaac tggcttgcag atccaaatgg tggaccggaa ggagaagagc
     1261 agattcgagg tgctttggct gaagctcgga aaatagcaga attatgtgat gatcctaaag
     1321 aaagagatga cattctacgt tcccttgggg aaatatctgc tctgacttct aaattagcag
     1381 atctacgaag acaggggaaa ggagattctc cagaggctcg agccttggcc aaacaggtgg
     1441 ccacggccct gcagaacctg cagaccaaaa ccaaccgggc tgtggccaac agcagaccgg
     1501 ccaaagcagc tgtacacctt gagggcaaga ttgagcaagc acagcggtgg attgataatc
     1561 ccacagtgga tgaccgtgga gtcggtcagg ctgccatccg ggggcttgtg gccgaagggc
     1621 atcgtctggc taatgttatg atggggcctt atcggcaaga tcttctcgcc aagtgtgacc
     1681 gagtggacca gctgacagcc cagctggctg acctggctgc cagaggggaa ggggagagtc
     1741 ctcaggcacg agcacttgca tctcagctcc aagactcctt aaaggatcta aaagctcgga
     1801 tgcaggaggc catgactcag gaagtgtcag atgttttcag cgataccaca actcccatca
     1861 agctgttggc agtggcagcc acggcgcctc ctgatgcgcc taacagggaa gaggtatttg
     1921 atgagagggc agctaacttt gaaaaccatt caggaaagct tggtgctacg gccgagaagg
     1981 cggctgcggt tggtactgct aataaatcaa cagtggaagg cattcaggcc tcagtgaaga
     2041 cggcccgaga actcacaccc caggtggtct cggctgctcg tatcttactt aggaaccctg
     2101 gaaatcaagc tgcttatgaa cattttgaga ccatgaagaa ccagtggatc gataatgttg
     2161 aaaaaatgac agggctggtg gacgaagcca ttgataccaa atctctgttg gatgcttcag
     2221 aagaagcaat taaaaaagac ctggacaagt gcaaggtagc tatggccaac attcagcctc
     2281 agatgctggt tgctggggca accagtattg ctcgtcgggc caaccggatc ctgctggtgg
     2341 ctaagaggga ggtggaaaat tccgaggatc ccaagttccg tgaggctgtg aaagctgcct
     2401 ctgatgaatt gagcaaaacc atctccccga tggtgatgga tgcaaaagct gtggctggaa
     2461 acatttccga ccctggactg caaaagagct tcctggactc aggatatcgg atcctgggag
     2521 ctgtggccaa ggtcagagaa gccttccaac ctcaggagcc tgacttcccg ccgcctccac
     2581 cagaccttga acaactccga ctaacagatg agcttgctcc tcccaaacca cctctgcctg
     2641 aaggtgaggt ccctccacct aggcctccac caccagagga aaaggatgaa gagttccctg
     2701 agcagaaggc cggggaggtg attaaccagc caatgatgat ggctgccaga cagctccatg
     2761 atgaagctcg caaatggtcc agcaagggca atgacatcat tgcagcagcc aagcgcatgg
     2821 ctctgctgat ggctgagatg tctcggctgg taagaggggg cagtggtacc aagcgggcac
     2881 tcattcagtg tgccaaggac atcgccaagg cctcagatga ggtgactcgg ttggccaagg
     2941 aggttgccaa gcagtgcaca gataaacgga ttagaaccaa cctcttacag gtatgtgagc
     3001 gaatcccaac cataagcacc cagctcaaaa tcctgtccac agtgaaggcc accatgctgg
     3061 gccggaccaa catcagtgat gaggagtctg agcaggccac agagatgctg gttcacaatg
     3121 cccagaacct catgcagtct gtgaaggaga ctgtgcggga agctgaagct gcttcaatca
     3181 aaattcgaac agatgctgga tttacactgc gctgggttag aaagactccc tggtaccagt
     3241 ccggtggcag cggaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     3301 ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc aacgtaaacg
     3361 ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga aaacagagta
     3421 tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc ctgaccactg
     3481 cattccatta cggcaacagg gtattcgcca aatatccaga caacatacaa gactatttta
     3541 agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa gacgggggca
     3601 tttgcaacgc cagaaacgac ataacaatgg aaggggacac tttctataat aaagttcgat
     3661 tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg ctgaaatggg
     3721 agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat attgagatgg
     3781 ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact tacaaagcta
     3841 aggagaaggg tgtcaagtta ccaggcgccc actttgtgga ccactgcatt gagattttaa
     3901 gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct cattctggat
     3961 tgcctgacaa tgccagacga taagcggccg cgactctaga tcataatcag ccataccaca
     4021 tttgtagaat ttgcc
//
