LOCUS       sequence-115256-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, R6K replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18R6K-mini-Tn7T-Gm
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(198..245)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(286..291,292..308,309..314)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gatgggaact gggtgtagcg tcgtaagcta atacgaaaat taaaaatgac aaaatagttt
       61 ggaactagat ttcacttatc tggttggcct gcaaggcctt cgcgaggtac cgggcccaag
      121 cttctcgagg aattcctgca gcccggggga tccactagtg agctcatgca tgatcgaatt
      181 agcttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
      241 acttcaagat cccctgattc cctttgtcaa cagcaatgga tcgaattgac ataagcctgt
      301 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      361 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      421 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      481 ttgatgttat ggagcagcaa cgatgttacg cagcagcaac gatgttacgc agcagggcag
      541 tcgccctaaa acaaagttag gtggctcaag tatgggcatc attcgcacat gtaggctcgg
      601 ccctgaccaa gtcaaatcca tgcgggctgc tcttgatctt ttcggtcgtg agttcggaga
      661 cgtagccacc tactcccaac atcagccgga ctccgattac ctcgggaact tgctccgtag
      721 taagacattc atcgcgcttg ctgccttcg
//
