LOCUS       sequence-115258-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, for construction of B-galactosidase protein 
            fusions, frame 3. See Depositor Comments below for information 
            regarding the lacZ sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Gm-LacZ30
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  60..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgcc caaacataac aggaagaaaa atgccccgct
       61 gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt aaggattatt
      121 tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta atacgaaaat
      181 taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct gcaaggaaat
      241 agtacataat ggatttcctt acgcgaaata cgggcagaca tggcctgccc ggttattatt
      301 atttttgaca ccagaccaac tggtaatggt agcgaccggc gctcagctgt aattccgccg
      361 atactgacgg gctccaggag tcgtcgccac caatccccat atggaaaccg tcgatattca
      421 gccatgtgcc ttcttccgcg tgcagcagat ggcgatggct ggtttccatc agttgctgtt
      481 gactgtagcg gctgatgttg aactggaagt cgccgcgcca ctggtgtggg ccataattca
      541 attcgcgcgt cccgcagcgc agaccgtttt cgctcgggaa gacgtacggg atatacatgt
      601 ctgacaatgg cagatcccag cggtcaaaac aggcggcagt aaggcggtcg ggatagtttt
      661 cttgcggccc taatccgagc cagtttaccc gctctgctac ctgcgccagc tggcagttca
      721 ggccaatccg cgccggatgc ggtgtatcgc tcgccacttc aacatcaacg gtaatcgcca
//
