LOCUS       sequence-115261-         549 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 luxCDABE transcriptional fusion vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Gm-lux
FEATURES             Location/Qualifiers
     source          1..549
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     189..205
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     190..206
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      234..328
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      431..517
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta aactagacag
       61 aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt
      121 atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg
      181 cgtggggttc gaggtcgacg gtatcgataa gctagcttaa ttagctgagc ttggactcct
      241 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      301 ccgccgggcg ttttttattg gtgagaatcc aagctagact gcgatgagtg gcagggcggg
      361 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggggtaat gactctctag
      421 cttgaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      481 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgctaggag cttgcggccc
      541 ggacgatcg
//
