LOCUS       sequence-115263-         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of 
            Staphylococcus aureus sortase A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA7
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(512..556)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(563..580)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(590..607)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             620..642
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aaaaaaatgt cgcacaatgt gcgccatttt tcacttcaca ggtcgtttaa acttatttga
       61 cttctgtagc tacaaagatt ttacgggttt cccaaacgcc tgtctcttca ttgtaatcat
      121 cacaagtaat taatgttaat tgtttatctt tacctttttg ttcatctaga acttctacgg
      181 ctgtaggctt aacgtttctt atacttgtca ttttatactt acgtgtttca ttaccaactt
      241 taaagtacac catactacct tttttggctg ctttaagatt tgtaaattga tagttcggac
      301 ggtcaatgaa agtgtgtcct gcaattgaaa tattttgatc atctagtgag gcattttcct
      361 ttgcaaagct tacacctcta tttaattgtt cccgtgttgc tggtcctgga tatactggtt
      421 ctttaatatc agcatctgga atttcaatat agcctgccac tttcgattta tctttcggaa
      481 tttgaggttt agcttgggta cccagatctg ggctgtccat gtgctggcgt tcgaatttag
      541 cagcagcggt ttctttcata ccagaaccgc gtggcaccag accagaagaa tgatgatgat
      601 gatggtggcc catggtatat ctccttctta aagttaaaca aaattattt
//
