LOCUS       sequence-115268-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable, for YFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp-eyfp
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      35..129
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      232..318
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(449..496)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(548..553,554..570,571..576)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ctgtacaagt aataagctta attagctgag cttggactcc tgttgataga tccagtaatg
       61 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      121 ggtgagaatc caagctagac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
      181 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
      241 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      301 ctcctgagta ggacaaatcc gccgctagga gcttgcggcc cccttcgcga ggtaccgggc
      361 ccaagcttct cgaggaattc ctgcagcccg ggggatccac tagtgagctc atgcatgatc
      421 gagctcgaat tagcttcaaa agcgctctga agttcctata ctttctagag aataggaact
      481 tcggaatagg aacttcaaga tcccctgatt ccctttgtca acagcaatgg ataattcacg
      541 aacccagttg acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc
      601 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
      661 catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca agcagcaagc
      721 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
      781 cagtcgccct
//
