LOCUS       sequence-11527-e        3923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Nachury MV, Loktev AV, Zhang Q, Westlake CJ, Peranen J, Merdes A, 
            Slusarski DC, Scheller RH, Bazan JF, Sheffield VC, Jackson PK
  TITLE     A core complex of BBS proteins cooperates with the GTPase Rab8 to 
            promote ciliary membrane biogenesis.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1201-13.
  PUBMED    17574030
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1201-13."
COMMENT     Sequence Label: pENTR-Rabin8
FEATURES             Location/Qualifiers
     source          1..3923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2048..2147)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2164..2183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2165..2183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2188..2204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2188..2204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2306..2325)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2317..3126
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2384..2403)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3273..3861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3762..3781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttgatatg gctaatgatc ccttggaagg cttccatgaa gtaaaccttg cttcacctac
      721 ttctccggac cttcttggtg tgtatgaatc aggaactcaa gagcagacta cctcaccaag
      781 tgtcatctac cggccacacc cttcagcttt atcctctgta cctatccagg caaatgcatt
      841 agatgtttct gaacttccta cacaacccgt gtattcatcc cccagacgtt taaattgtgc
      901 ggaaatatct agtatcagct ttcatgttac agacccagcc ccttgctcta cctctggagt
      961 cacagctgga ttaactaaat taactacaag aaaggacaac tataatgcag agagagagtt
     1021 tttacagggt gctactataa cagaggcttg cgatggcagt gatgatattt ttgggttgag
     1081 tactgatagt ctgtctcgtt tacgaagccc atctgttttg gaagttagag aaaagggcta
     1141 tgaacgatta aaagaagaac tcgcaaaagc tcagagggaa ctgaagttaa aagatgaaga
     1201 atgtgagagg ctttcaaaag tgcgagatca acttggacag gaattggaag aactcacagc
     1261 tagtctattt gaggaagctc ataaaatggt gagagaagca aatatcaagc aggcaacagc
     1321 agaaaaacag ctaaaagaag cacaaggaaa aattgatgta cttcaagctg aagtagctgc
     1381 attgaagaca cttgtattgt ccagttctcc aacatcacct acgcaggagc ctttgccagg
     1441 tggaaagaca ccttttaaaa aggggcatac aagaaataaa agcacaagca gtgctatgag
     1501 tggcagtcat caggacctca gtgtgataca gccaattgta aaagactgca aagaggctga
     1561 cttatccttg tataatgaat tccgattgtg gaaggatgag cccacaatgg acaggacgtg
     1621 tcctttctta gacaaaatct accaggaaga tatctttcca tgtttaacat tctcaaaaag
     1681 tgagttggct tcagctgttc tggaggctgt ggaaaacaat actctaagca ttgaaccagt
     1741 gggattacaa cctatccggt ttgtgaaagc ttctgcagtt gaatgcggag gaccaaaaaa
     1801 atgtgctctc actggccaga gtaagtcctg taaacacaga attaaattag gggactcaag
     1861 caactattat tatatttctc ctttttgcag atacaggatc acttctgtat gtaacttttt
     1921 tacatacatt cgatacattc agcagggact cgtgaaacag caggatgttg atcagatgtt
     1981 ttgggaggtt atgcagttga gaaaagagat gtcattggca aagctgggtt atttcaaaga
     2041 ggaactctag caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2101 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2161 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2221 tctcaaaatc tctgatgtta cattgcacaa gataaaataa tatcatcatg aacaataaaa
     2281 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2341 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2401 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2461 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2521 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2581 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2641 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2701 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2761 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2821 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2881 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2941 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3001 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3061 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3121 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3181 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3901 tgctggcctt ttgctcacat gtt
//
