LOCUS       sequence-115271-         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus 
            aureus sortase A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA5
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(457..501)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(508..525)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(535..552)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             565..587
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tttgacttct gtagctacaa agattttacg ggtttcccaa acgcctgtct cttcattgta
       61 atcatcacaa gtaattaatg ttaattgttt atctttacct ttttgttcat ctagaacttc
      121 tacggctgta ggcttaacgt ttcttatact tgtcatttta tacttacgtg tttcattacc
      181 aactttaaag tacaccatac tacctttttt ggctgcttta agatttgtaa attgatagtt
      241 cggacggtca atgaaagtgt gtcctgcaat tgaaatattt tgatcatcta gtgattcgtt
      301 ttcttctgca aagcttacac ctctatttaa ttgttcccgt gttgctggtc ctggatatac
      361 tggttcttta atatcagcat ctggaatttc aatatagcct gccactttcg atttatcttt
      421 cggaatttga ggtttagctt gggtacccag atctgggctg tccatgtgct ggcgttcgaa
      481 tttagcagca gcggtttctt tcataccaga accgcgtggc accagaccag aagaatgatg
      541 atgatgatgg tggcccatgg tatatctcct tcttaaagtt aaacaaaaa
//
