LOCUS       sequence-115272-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp-gfpmut3
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..23
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     mobile_element  117..282
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            441..549
                     /note="incP origin of transfer"
ORIGIN
        1 cgcatgctta atttctcctc tttaattcta gagtggtgtc gcattatagg gagttattcc
       61 ggcctgacta gaggaaattt aaaataattt tctgaccgcg caacgccttg caggccaacc
      121 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg
      181 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc
      241 atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc
      301 tgttatgttt gggcgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      361 attgggcgct cttccgcttc ctcgctcact gacccgctgc gctcggtcgt tcggctgcgg
      421 cgagcggtat cagagcttat cggccagcct cgcagagcag gattcccgtt gagcaccgcc
      481 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
      541 atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa
      601 aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg
      661 aagcagggtt atgcagcgga aagtatacct taaggaatcc cctgataacg ca
//
