LOCUS       sequence-115274-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  source of CmR FRT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFCM1
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    253..300
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(386..402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(386..402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(399..421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    410..426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(434..440,441..458,459..464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    479..500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(617..634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
       61 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt
      121 aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc
      181 ggatgaatgg cagaaattcg aaagcaaatt cgacccatcc attgctgttg acaaagggaa
      241 tcaggggatc ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
      301 agagcgcttt tgaagctaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
      361 ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      421 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      481 gagtgagcta actcacatta attgcgttgc cctcactgcc cgctttccag tcgggaagcc
      541 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      601 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      661 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatca
//
