LOCUS       sequence-115275-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flp recombinase expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFLP3
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             142..150
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cgacgtgcgt cctcaagctg ctcttgtgtt aatggtttct tttttgtgct catacgttaa
       61 atctatcacc gcaagggata aatatctaac accgtgcgtg ttgactattt tacctctggc
      121 ggtgataatg gttgcatgta ctaaggaggt tgtatgccac aatttgatat attatgtaaa
      181 acaccaccta aggtgcttgt tcgtcagttt gtggaaaggt ttgaaagacc ttcaggtgag
      241 aaaatagcat tatgtgctgc tgaactaacc tatttatgtt ggatgattac acataacgga
      301 acagcaatca agagagccac attcatgagc tataatacta tcataagcaa ttcgctgagt
      361 ttcgatattg tcaataaatc actccagttt aaatacaaga cgcaaaaagc aacaattctg
      421 gaagcctcat taaagaaatt gattcctgct tgggaattta caattattcc ttactatgga
      481 caaaaacatc aatctgatat cactgatatt gtaagtagtt tgcaattaca gttcgaatca
      541 tcggaagaag cagataaggg aaatagccac agtaaaaaaa tgcttaaagc acttctaagt
      601 gagggtgaaa gcatctggga gatcactgag aaaatactaa attcgtttga gtatacttcg
      661 agatttacaa aaacaaaaac tttataccaa ttcctcttcc tagctacttt catcaattgt
      721 ggaagattca gcgatattaa gaacgttgat
//
