LOCUS       sequence-115279-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 vector with Ptac expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18-mini-Tn7T-LAC
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     234..250
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     235..251
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      279..373
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      476..562
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    608..655
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga
       61 acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg
      121 aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac
      181 tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt
      241 cgacggtatc gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt
      301 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
      361 tattggtgag aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa
      421 ggcagttatt ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat
      481 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      541 cgctctcctg agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg
      601 ggatcttgaa gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc
      661 gcttttgaag ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc
      721 ggcgttgtga c
//
