LOCUS       sequence-115282-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA2
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             103..147
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     rep_origin      695..1075
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(782..801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     992..1013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaatctaga aataattttg tttaacttta agaaggagat ataccatggg ccaccatcat
       61 catcatcatt cttctggtct ggtgccacgc ggttctggta tgaaagaaac cgctgctgct
      121 aaattcgaac gccagcacat ggacagccca gatctgggta cccaagctaa acctcaaatt
      181 ccgaaagata aatcgaaagt ggcaggctat attgaaattc cagatgctga tattaaagaa
      241 ccagtatatc caggaccagc aacacctgaa caattaaata gaggtgtaag ctttgcaaag
      301 gaaaatgcct cactagatga tcaaaatatt tcaattgcag gacacacttt cattgaccgt
      361 ccgaactatc aatttacaaa tcttaaagca gccaaaaaag gtagtatggt gtactttaaa
      421 gttggtaatg aaacacgtaa gtataaaatg acaagtataa gagatgttaa gcctacagat
      481 gtagaagttc tagatgaaca aaaaggtaaa gataaacaat taacattaat tacttgtgat
      541 gattacaatg aaaagacagg cgtttgggaa aaacgtaaaa tctttgtagc tacagaagtc
      601 aaataagttt aaacgacctg tgaagtgaaa aatggcgcac attgtgcgac attttttttg
      661 tctgccgttt accgctactg cgtcacggat ctccacgcgc cctgtagcgg cgcattaagc
      721 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      781 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      841 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
      901 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      961 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     1021 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     1081 tggttaaaat ga
//
