LOCUS       sequence-115285-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A transformation-associated recombination (TAR) based genomic 
            capture and expression vector for antibiotic production in B. 
            subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Li Y, Li Z, Yamanaka K, Xu Y, Zhang W, Vlamakis H, Kolter R, Moore 
            BS, Qian PY
  TITLE     Directed natural product biosynthesis gene cluster capture and 
            expression in the model bacterium Bacillus subtilis.
  JOURNAL   Sci Rep. 2015 Mar 24;5:9383. doi: 10.1038/srep09383.
  PUBMED    25807046
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-03-24"; volume: "5:9383. doi"; pages: " 
            10.1038/srep09383"
COMMENT     Sequence Label: pCAPB02
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..232
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             233..907
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 gtaatatatg tgtactttgc agttatgacg ccagatggca gtagtggaag atattcttta
       61 ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg gagcttttct ttttttgccg
      121 attaagaatt aattcggtag aaaaaagaaa aggagagggc caagagggag ggcattggtg
      181 actattgagc acgtgagtat acgtgattaa gcacacaaag gcagcttgga gtatgtctgt
      241 tattaatttc acaggtagtt ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga
      301 ggccgcagaa tgtgctctag attccgatgc tgacttgctg ggtattatat gtgtgcccaa
      361 tagaaagaga acaattgacc cggttattgc aaggaaaatt tcaagtcttg taaaagcata
      421 taaaaatagt tcaggcactc cgaaatactt ggttggcgtg tttcgtaatc aacctaagga
      481 ggatgttttg gctctggtca atgattacgg cattgatatc gtccaactgc atggagatga
      541 gtcgtggcaa gaataccaag agttcctcgg tttgccagtt attaaaagac tcgtatttcc
      601 aaaagactgc aacatactac tcagtgcagc ttcacagaaa cctcattcgt ttattccctt
      661 gtttgattca gaagcaggtg ggacaggtga acttttggat tggaactcga tttctgactg
      721 ggttggaagg caagagagcc ccgaaagctt acattttatg ttagctggtg gactgacgcc
      781 agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt ggtgttgatg taagcggagg
      841 tgtggagaca aatggtgtaa aagactctaa caaaatagca aatttcgtca aaaatgctaa
      901 gaaataggtt attactgagt agtatttatt taagtattgt ttgtgcactt gccgatctat
      961 gcggtgtgaa ataccgcac
//
