LOCUS       sequence-115287-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A transformation-associated recombination (TAR) based genomic 
            capture and expression vector for antibiotic production in B. 
            subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Li Y, Li Z, Yamanaka K, Xu Y, Zhang W, Vlamakis H, Kolter R, Moore 
            BS, Qian PY
  TITLE     Directed natural product biosynthesis gene cluster capture and 
            expression in the model bacterium Bacillus subtilis.
  JOURNAL   Sci Rep. 2015 Mar 24;5:9383. doi: 10.1038/srep09383.
  PUBMED    25807046
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-03-24"; volume: "5:9383. doi"; pages: " 
            10.1038/srep09383"
COMMENT     Sequence Label: pCAPB02
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    283..299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(403..437)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(517..544)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(563..649)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaatcgtctc cctccgtttg aatatttgat tgatcgtaac cagatgaagc actctttcca
       61 ctatccctac agtgttatgg cttgaacaat cacgaaacaa taattggtac gtacgatctt
      121 tcagccgact caaacatcaa atcttacaaa tgtagtcttt gaaagtatta catatgtaag
      181 atttaaatgc aaccgttttt tcggaaggaa atgatgacct cgtttccacc gaattagctt
      241 gcatgcatcg atgcggccgc tccgcgggtc gactaagctt aattgttatc cgctcacaat
      301 tacacacatt atgccacacc ttgtagataa agtcaacaac ttttgcaaaa tgaattgtga
      361 gtgctcacat ttaccctcga gcaacgttct tgccattgct gcataaaaaa cgcccggcgg
      421 caaccgagcg ttctgaatta attaatcatc gggaagatct tcatcaccga aacgcggcag
      481 gcagctctag agttaacaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
      541 ttctgcttag ctagagcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
      601 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctcaagc
      661 tagaggtcga attcctgcag ctggcgaatg gcgattttcg ttcgtgaata catgttataa
      721 taactataac taataacgta acgtgactgg caagagatat ttttaaaaca atgaataggt
      781 ttacacttac tttagtttta tggaaatgaa agatcatatc atatataatc tagaataaaa
      841 ttaactaaaa taattattat ctagataa
//
