LOCUS       sequence-115289-        1196 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human codon-optimised TEV protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Oberst A, Pop C, Tremblay AG, Blais V, Denault JB, Salvesen GS, 
            Green DR
  TITLE     Inducible dimerization and inducible cleavage reveal a requirement 
            for both processes in caspase-8 activation.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16632-42. doi: 
            10.1074/jbc.M109.095083. Epub 2010 Mar 22.
  PUBMED    20308068
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.095083"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16632-42"
COMMENT     Sequence Label: pcDNA3.1-V5-hTEV
FEATURES             Location/Qualifiers
     source          1..1196
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             158..199
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             200..907
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     CDS             977..1018
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1028..1045
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1068..1085)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1074..1185
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      121 tccactagtc cagtgtggtg gaattgccct taccatgggc aaacctattc ctaatccact
      181 gctcggactc gacagcacag gcgagagcct cttcaaggga cccagggact acaaccccat
      241 tagcagcacc atctgccacc tcaccaatga gtccgacggc cataccacca gcctgtatgg
      301 aatcggcttt ggacctttca tcatcaccaa caagcatctg tttcggcgga acaacggcac
      361 cctgctggtg cagagcctgc atggcgtgtt caaggtcaag aacacaacta ccctccagca
      421 gcacctgatc gatggaagag acatgatcat catccggatg ccaaaagact ttccaccttt
      481 tccccaaaaa ctcaaattcc gggagcctca gagagaagag cggatctgtc tggtgaccac
      541 aaatttccag acaaaaagca tgagcagcat ggtgtccgat acaagctgca cattcccatc
      601 cagcgatggc atcttctgga aacattggat tcagacaaaa gatggccagt gcggaagccc
      661 actcgtctcc actagagacg ggttcatcgt ggggattcac agcgctagca attttacaaa
      721 taccaataat tactttacat ccgtcccaaa gaacttcatg gagctgctga caaaccagga
      781 agcacagcag tgggtgagcg gctggaggct gaacgccgat tccgtcctgt ggggcggcca
      841 caaggtcttc atggtgaagc ccgaagagcc tttccagcca gtgaaggagg ccactcaact
      901 gatgaactaa aagggcaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
      961 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     1021 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
     1081 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1141 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctga
//
