LOCUS       sequence-115291-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane , Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-UbiquitinC-N-18
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..249
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     regulatory      298..307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(449..467)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     717..736
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcagatccg ctagcgccac catgcagatc ttcgtgaaga ctctgactgg taagaccatc
       61 accctcgagg ttgagcccag tgacaccatt gagaatgtca aggcaaagat ccaagataag
      121 gaaggcatcc ctcctgacca gcagaggctg atctttgctg gaaaacagct ggaagatggg
      181 cgcaccctgt ctgactacaa catccagaaa gagtccaccc tgcacctggt actccgtctc
      241 agaggtgggg gtggaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc
      301 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      361 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      421 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      481 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      541 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      601 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      661 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      721 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      781 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
//
