LOCUS       sequence-115292-         266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the wild type of Staphylococcus aureus sortase A in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA
FEATURES             Location/Qualifiers
     source          1..266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..39
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             46..90
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 catcatcatc attcttctgg tctggtgcca cgcggttctg gtatgaaaga aaccgctgct
       61 gctaaattcg aacgccagca catggacagc ccagatctgg gtacccaagc taaacctcaa
      121 attccgaaag ataaatcgaa agtggcaggc tatattgaaa ttccagatgc tgatattaaa
      181 gaaccagtat atccaggacc agcaacacct gaacaattaa atagaggtgt aagctttgca
      241 gaagaaaacg aatcactaga tgatca
//
