LOCUS       sequence-115294-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express the 
            ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under 
            intersectional control by Dre and Cre recombinases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..67)
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atccagaggt tgattatcga taagcgacta gtgataactt cgtatagcat acattatagc
       61 aatttatttg gcgcgccatt taaatcctgg ccggccttgc caccatggat tatggaggcg
      121 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa cccagtagtc gtcaatggct
      181 ctgtacttgt gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa
      241 acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
      301 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg
      361 tgtgcgctat cgagatggtc aaggtgattc tcgagttctt cttcgagttt aagaacccgt
      421 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc gagtggcttc
      481 tcacctgccc ggtcattctc attcgcctgt caaacctgac gggcttgtcc aacgactaca
      541 gcaggcgcac catgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt
      601 ccgccatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
      661 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccacacc gtgccgaagg
      721 gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
      781 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg tacggct
//
