LOCUS       sequence-115295-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of 
            Staphylococcus aureus sortase A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA7woH
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..93
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     rep_origin      641..999
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(728..747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     938..959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaatctaga aataattttg tttaacttta agaaggagat ataccatgaa agaaaccgct
       61 gctgctaaat tcgaacgcca gcacatggac agcccagatc tgggtaccca agctaaacct
      121 caaattccga aagataaatc gaaagtggca ggctatattg aaattccaga tgctgatatt
      181 aaagaaccag tatatccagg accagcaaca cgggaacaat taaatagagg tgtaagcttt
      241 gcaaaggaaa atgcctcact agatgatcaa aatatttcaa ttgcaggaca cactttcatt
      301 gaccgtccga actatcaatt tacaaatctt aaagcagcca aaaaaggtag tatggtgtac
      361 tttaaagttg gtaatgaaac acgtaagtat aaaatgacaa gtataagaaa cgttaagcct
      421 acagccgtag aagttctaga tgaacaaaaa ggtaaagata aacaattaac attaattact
      481 tgtgatgatt acaatgaaga gacaggcgtt tgggaaaccc gtaaaatctt tgtagctaca
      541 gaagtcaaat aagtttaaac gacctgtgaa gtgaaaaatg gcgcacattg tgcgacattt
      601 tttttgtctg ccgtttaccg ctactgcgtc acggatctcc acgcgccctg tagcggcgca
      661 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      721 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      781 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      841 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      901 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
      961 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatt
//
