LOCUS       sequence-115300-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Fatscher T, Boehm V, Weiche B, Gehring NH
  TITLE     The interaction of cytoplasmic poly(A)-binding protein with 
            eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA 
            decay.
  JOURNAL   RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014
            Aug 21.
  PUBMED    25147240
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.044933.114"; journalName: "RNA"; date: "2014-10"; 
            volume: "20"; issue: "10"; pages: "1579-92"
COMMENT     Sequence Label: pCI-MS2V5-PABPC1 MLLEmut
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             51..437
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             444..485
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gctcttaagg ctagagtact taatacgact cactataggc tagcaccatg gcttctaact
       61 ttactcagtt cgttctcgtg gacaatggcg gaactggcga cgtgactgtc gccccaagca
      121 acttcgctaa cggggtcgct gaatggatca gctctaactc gcgttcacag gcttacaaag
      181 taacctgtag cgttcgtcag agctctgcgc agaatcgcaa atacaccatc aaagtcgagg
      241 tgcctaaagt ggcaacccag actgttggtg gtgtagagct tcctgtagcc gcatggcgtt
      301 cgtacttaaa tatggaacta accattccaa ttttcgccac gaactccgac tgcgagctta
      361 ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc gattccctca gcaatcgcag
      421 caaactccgg catctacggc ggtggcaagc ccatccctaa cccactgctg ggcctggaca
      481 gcaccctcga gaaccccagt gcccccagct acccgatggc ctcgctctac gtgggggacc
      541 tccaccccga cgtgaccgag gcgatgctct acgagaagtt cagcccggcc gggcccatcc
      601 tctccatccg ggtctgcagg gacatgatca cccgccgctc cttgggctac gcgtatgtga
      661 acttccagca gccggcggac gcggagcgtg ctttggacac catgaatttt gatgttataa
      721 agggcaagcc agtacgcatc atgtggtctc agcgtgatcc atcacttcgc
//
