LOCUS       sequence-115302-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of 
            Staphylococcus aureus sortase A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA7woH
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(510..554)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     RBS             564..586
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        625..680
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    626..644
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    656..674
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 aaaaatgtcg cacaatgtgc gccatttttc acttcacagg tcgtttaaac ttatttgact
       61 tctgtagcta caaagatttt acgggtttcc caaacgcctg tctcttcatt gtaatcatca
      121 caagtaatta atgttaattg tttatcttta cctttttgtt catctagaac ttctacggct
      181 gtaggcttaa cgtttcttat acttgtcatt ttatacttac gtgtttcatt accaacttta
      241 aagtacacca tactaccttt tttggctgct ttaagatttg taaattgata gttcggacgg
      301 tcaatgaaag tgtgtcctgc aattgaaata ttttgatcat ctagtgaggc attttccttt
      361 gcaaagctta cacctctatt taattgttcc cgtgttgctg gtcctggata tactggttct
      421 ttaatatcag catctggaat ttcaatatag cctgccactt tcgatttatc tttcggaatt
      481 tgaggtttag cttgggtacc cagatctggg ctgtccatgt gctggcgttc gaatttagca
      541 gcagcggttt ctttcatggt atatctcctt cttaaagtta aacaaaatta tttctagatt
      601 tttgtcgaac tattcatttc acttttctct atcactgata gggagtggta aaataactct
      661 atcaatgata gagtgtcaac aaaaattagg aattaatcat ctggccattc gatggtgtcg
      721 ggtcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggcag aattacaac
//
