LOCUS       sequence-115305-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs 
            containing MS2 stem loops..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Fatscher T, Boehm V, Weiche B, Gehring NH
  TITLE     The interaction of cytoplasmic poly(A)-binding protein with 
            eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA 
            decay.
  JOURNAL   RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014
            Aug 21.
  PUBMED    25147240
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.044933.114"; journalName: "RNA"; date: "2014-10"; 
            volume: "20"; issue: "10"; pages: "1579-92"
COMMENT     Sequence Label: pCI-MS2V5-PABPC1
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        11..29
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             41..427
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             434..475
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctagagtact taatacgact cactataggc tagcaccatg gcttctaact ttactcagtt
       61 cgttctcgtg gacaatggcg gaactggcga cgtgactgtc gccccaagca acttcgctaa
      121 cggggtcgct gaatggatca gctctaactc gcgttcacag gcttacaaag taacctgtag
      181 cgttcgtcag agctctgcgc agaatcgcaa atacaccatc aaagtcgagg tgcctaaagt
      241 ggcaacccag actgttggtg gtgtagagct tcctgtagcc gcatggcgtt cgtacttaaa
      301 tatggaacta accattccaa ttttcgccac gaactccgac tgcgagctta ttgttaaggc
      361 aatgcaaggt ctcctaaaag atggaaaccc gattccctca gcaatcgcag caaactccgg
      421 catctacggc ggtggcaagc ccatccctaa cccactgctg ggcctggaca gcaccctcga
      481 gaaccccagt gcccccagct acccgatggc ctcgctctac gtgggggacc tccaccccga
      541 cgtgaccgag gcgatgctct acgagaagtt cagcccggcc gggcccatcc tctccatccg
      601 ggtctgcagg gacatgatca cccgccgctc cttgggctac gcgtatgtga acttccagca
      661 gccggcggac gcggagcgtg ctttggacac catgaatttt gatgttataa agggcaagcc
      721 agtacgcatc atgtggtctc agcgtgatcc atcacttcgc
//
