LOCUS       sequence-115309-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus 
            aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA5woH
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(642..661)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggacagccca gatctgggta cccaagctaa acctcaaatt ccgaaagata aatcgaaagt
       61 ggcaggctat attgaaattc cagatgctga tattaaagaa ccagtatatc caggaccagc
      121 aacacgggaa caattaaata gaggtgtaag ctttgcagaa gaaaacgaat cactagatga
      181 tcaaaatatt tcaattgcag gacacacttt cattgaccgt ccgaactatc aatttacaaa
      241 tcttaaagca gccaaaaaag gtagtatggt gtactttaaa gttggtaatg aaacacgtaa
      301 gtataaaatg acaagtataa gaaacgttaa gcctacagcc gtagaagttc tagatgaaca
      361 aaaaggtaaa gataaacaat taacattaat tacttgtgat gattacaatg aagagacagg
      421 cgtttgggaa acccgtaaaa tctttgtagc tacagaagtc aaataagttt aaacgacctg
      481 tgaagtgaaa aatggcgcac attgtgcgac attttttttg tctgccgttt accgctactg
      541 cgtcacggat ctccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      601 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      661 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      721 agggttccga tttagtgctt tacggcacc
//
