LOCUS       sequence-115314-        6694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An amino-terminal mCerulean fragment was fused to RGS7. When 
            co-expressed with a carboxyl terminal fragment of CFP fused to 
            Gbeta-5, a fluorescent signal is produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: Cer(1-158)-RGS7
FEATURES             Location/Qualifiers
     source          1..6694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     471..490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(603..1061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(740..761)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     955..974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(1186..1204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1272..1376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1377..1445,1446..2237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1595..1614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        2310..2689
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2690..2893
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2844..2864
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2938..2957
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2938..2956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2988..2997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3039..3060)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3300..3319)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(4966..4984)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4967..4984)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          5068..5133
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5263..5283
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5555..5689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5592..5611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5646..5665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6535..6670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
       61 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
      601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      661 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
      721 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      781 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
      841 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     1081 gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     1141 aagggatttt agacaggaac ggtacgccag ctggattacc aaagggcctc gtgatacgcc
     1201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     1621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1921 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2281 aacttcattt ttaatttctc tagcgcgttg acattgatta ttgactagtt attaatagta
     2341 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2401 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2461 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt
     2521 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2581 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2641 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2701 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2761 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2821 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2881 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     2941 tacgactcac tatagggaga cccaagcttg gtaccgagct cggatccgcc accatggtga
     3001 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3061 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3121 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3181 ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     3241 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3301 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3361 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3421 agtacaacgc catcagcgac aacgtctata tcaccgccga caagcagaga tccatcgcaa
     3481 ccatggccca ggggaataat tatgggcaga ccagcaacgg ggtggccgat gaatcaccca
     3541 acatgctggt gtacagaaag atggaagacg tcatagcacg gatgcaagat gaaaaaaatg
     3601 gaattcctat tcgtacggtc aaaagctttc tttccaagat acctagcgtc ttctctggtt
     3661 cagacattgt tcaatggttg ataaagaact taactataga agatccagtg gaggcgctcc
     3721 atttgggaac attaatggct gcccacggct acttctttcc aatctcagat catgtcctca
     3781 cactcaagga tgatggcacc ttttaccggt ttcaaacccc ctatttttgg ccatcaaatt
     3841 gttgggagcc ggaaaacaca gattatgccg tttacctctg caagagaaca atgcaaaaca
     3901 aggcacgact ggagctcgca gactatgagg ctgagagcct ggccaggctg cagagagcat
     3961 ttgcccggaa gtgggagttc attttcatgc aagcagaagc acaagcaaaa gtggacaaga
     4021 agagagacaa gattgaaagg aagatccttg acagccaaga gagagcgttc tgggacgtgc
     4081 acaggcccgt gcctggatgt gtaaatacaa ctgaagtgga cattaagaag tcatccagaa
     4141 tgagaaaccc ccacaaaaca cggaagtctg tctatggttt acaaaatgat attagaagtc
     4201 acagtcctac ccacacaccc acaccagaaa ctaaacctcc aacagaagat gagttacaac
     4261 aacagataaa atattggcaa atacagttag atagacatcg gttaaaaatg tcaaaagtcg
     4321 ctgacagtct actaagttac acggaacagt atttagaata cgacccgttt cttttgccac
     4381 ctgacccttc taacccatgg ctgtccgatg acaccacttt ctgggaactt gaggcaagca
     4441 aagaaccgag ccagcagagg gtaaaacgat ggggttttgg catggacgag gcattgaaag
     4501 acccagttgg gagagaacag ttccttaaat ttctagagtc agaattcagc tcggaaaatt
     4561 taagattctg gctggcagtg gaggacctga aaaagaggcc tattaaagaa gtaccctcaa
     4621 gagttcagga aatatggcaa gagtttctgg ctcccggagc ccccagtgct attaacttgg
     4681 attccaagag ttatgacaaa accacacaga acgtgaagga acctggacga tacacatttg
     4741 aagatgctca ggagcacatt tacaaactga tgaaaagtga ttcataccca cgttttataa
     4801 gatccagtgc ctatcaggag cttctacagg caaagaaaaa gggcagaaac atccctattt
     4861 tcccatgcca taaaaactgt acaccaacac tgagggccag cactaacctg ttatgaagat
     4921 ctatcacact ggcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct
     4981 aaatgctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     5041 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     5101 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     5161 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     5221 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     5281 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     5341 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     5401 tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga tcataatcag
     5461 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     5521 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     5581 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5641 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca tcccgccatg
     5701 gtatcaacgc catatttcta tttacagtag ggacctcttc gttgtgtagg taccgctgta
     5761 ttcctaggga aatagtagag gcaccttgaa ctgtctgcat cagccatata gcccccgctg
     5821 ttcgacttac aaacacaggc acagtactga caaacccata cacctcctct gaaataccca
     5881 tagttgctag ggctgtctcc gaactcatta caccctccaa agtcagagct gtaatttcgc
     5941 catcaagggc agcgagggct tctccagata aaatagcttc tgccgagagt cccgtaaggg
     6001 tagacacttc agctaatccc tcgatgaggt ctactagaat agtcagtgcg gctcccattt
     6061 tgaaaattca cttacttgat cagcttcaga agatggcgga gggcctccaa cacagtaatt
     6121 ttcctcccga ctcttaaaat agaaaatgtc aagtcagtta agcaggaagt ggactaactg
     6181 acgcagctgg ccgtgcgaca tcctctttta attagttgct aggcaacgcc ctccagaggg
     6241 cgtgtggttt tgcaagagga agcaaaagcc tctccaccca ggcctagaat gtttccaccc
     6301 aatcattact atgacaacag ctgttttttt tagtattaag cagaggccgg ggacccctgg
     6361 gcccgcttac tctggagaaa aagaagagag gcattgtaga ggcttccaga ggcaacttgt
     6421 caaaacagga ctgcttctat ttctgtcaca ctgtctggcc ctgtcacaag gtccagcacc
     6481 tccatacccc ctttaataag cagtttggga acgggtgcgg gtcttactcc gcccatcccg
     6541 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6601 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6661 tttggaggcc taggcttttg caaaaagcta attc
//
