LOCUS       sequence-115316-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus 
            aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA5woH
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(511..555)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     RBS             565..587
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        626..681
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    627..645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    657..675
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 aaaaaatgtc gcacaatgtg cgccattttt cacttcacag gtcgtttaaa cttatttgac
       61 ttctgtagct acaaagattt tacgggtttc ccaaacgcct gtctcttcat tgtaatcatc
      121 acaagtaatt aatgttaatt gtttatcttt acctttttgt tcatctagaa cttctacggc
      181 tgtaggctta acgtttctta tacttgtcat tttatactta cgtgtttcat taccaacttt
      241 aaagtacacc atactacctt ttttggctgc tttaagattt gtaaattgat agttcggacg
      301 gtcaatgaaa gtgtgtcctg caattgaaat attttgatca tctagtgatt cgttttcttc
      361 tgcaaagctt acacctctat ttaattgttc ccgtgttgct ggtcctggat atactggttc
      421 tttaatatca gcatctggaa tttcaatata gcctgccact ttcgatttat ctttcggaat
      481 ttgaggttta gcttgggtac ccagatctgg gctgtccatg tgctggcgtt cgaatttagc
      541 agcagcggtt tctttcatgg tatatctcct tcttaaagtt aaacaaaatt atttctagat
      601 ttttgtcgaa ctattcattt cacttttctc tatcactgat agggagtggt aaaataactc
      661 tatcaatgat agagtgtcaa caaaaattag gaattaatca tctggccatt cga
//
