LOCUS       sequence-115321-         450 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express the 
            ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under 
            intersectional control by Flp and Cre recombinases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..450
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..118
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(133..166)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    326..374
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     399..418
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ggccgccggc aagtgaattc aggccggcca tttaaatggc gcgccaccgg tgctagctcc
       61 ggagtttaaa caaggtcgac gcagataact tcgtataatg tatgctatac gaacggtaaa
      121 gttaacctcg aggaagttcc tatactttct agagaatagg aacttccacg gcctgccgcc
      181 ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg tggggaaaag
      241 gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac agagtgccag
      301 ccctgggacc gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg ttttattctg
      361 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
      421 tagcctcccc cctactcaat tagctgaggc
//
