LOCUS       sequence-115326-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral introduction of Cas9 into mammalian cell line..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Shi J, Wang E, Milazzo JP, Wang Z, Kinney JB, Vakoc CR
  TITLE     Discovery of cancer drug targets by CRISPR-Cas9 screening of protein
            domains.
  JOURNAL   Nat Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235.
  PUBMED    25961408
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235."
COMMENT     Sequence Label: MSCV_Cas9_puro
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             51..74
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             81..101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattaatg gattacaaag
       61 acgatgacga taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag
      121 cagccgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg
      181 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
      241 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
      301 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
      361 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
      421 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
      481 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
      541 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
      601 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg
      661 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
      721 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
      781 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa
      841 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
      901 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
      961 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca
     1021 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggc
//
