LOCUS       sequence-115328-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses intein-Cas9(S219) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Davis KM, Pattanayak V, Thompson DB, Zuris JA, Liu DR
  TITLE     Small molecule-triggered Cas9 protein with improved genome-editing 
            specificity.
  JOURNAL   Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. 
            Epub 2015 Apr 6.
  PUBMED    25848930
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1793"; journalName: "Nat Chem Biol"; date: 
            "2015-05"; volume: "11"; issue: "5"; pages: "316-8"
COMMENT     Sequence Label: pKMD106e - intein-Cas9(S219)
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttctggagtg tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat
       61 ccaccgagcc ctggacaaga tcacggacac tttgatccac ctgatggcca aggcaggcct
      121 gaccctgcag cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag
      181 gcacatgagt aacaaaggaa tggagcatct gtacagcatg aagtacaaga acgtggtgcc
      241 cctctatgac ctgctgctgg agatgctgga cgcccaccgc ctacatgcgg gtggttctgg
      301 tgctagccgc gtgcaggcgt tcgcggatgc cctggatgac aaattcctgc acgacatgct
      361 ggcggaagga ctccgctatt ccgtgatccg agaagtgctg ccaacgcggc gggcacgaac
      421 gttcgacctc gaggtcgagg aactgcacac cctcgtcgcc gaaggggttg tcgtgcacaa
      481 ctgccgacgg ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt
      541 cggtaacctt atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt
      601 agctgaagat gccaaattgc agcttagtaa ggacacgtac gatgacgatc tcgacaatct
      661 actggcacaa attggagatc agtatgcgga cttatttttg gctgccaa
//
