LOCUS       sequence-115340-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid: phaA, phaB, phaC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Kocharin K, Chen Y, Siewers V, Nielsen J
  TITLE     Engineering of acetyl-CoA metabolism for the improved production of 
            polyhydroxybutyrate in Saccharomyces cerevisiae.
  JOURNAL   AMB Express. 2012 Sep 25;2(1):52. doi: 10.1186/2191-0855-2-52.
  PUBMED    23009357
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2012-09-25"; volume: "2(1):52. doi"; pages: " 
            10.1186/2191-0855-2-52"
COMMENT     Sequence Label: pKK01
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..91
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(190..379)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(409..438)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
       61 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      121 gcctctcccc gcgcgttggc cgattcatta atgcagctgg ataaacgccg gcgaaacgat
      181 ccggaggatc ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca
      241 tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga aatataaata acgttcttaa
      301 tactaacata actataaaaa aataaatagg gacctagact tcaggttgtc taactccttc
      361 cttttcggtt agagcggatc ttagctagcc gcggtaccaa gcttactcga ggtcttcttc
      421 ggaaatcaac ttctgttcca tgtcgaccta acccatgtgt agaccaccgt ttaaggagaa
      481 atctgcacct gtactaaaac ccgattcctc actggaaagc caagcgcaaa ttgaagctat
      541 ttcttcaggc aaacctaacc tcttaactgg tatagtggcg acaatcttgt ctagaacgtc
      601 ttgcctaatt gccttcacca tatcggttgc aatatatcct ggggagactg tatttacagt
      661 tacacctttc gttgcaactt cttgagccaa tgccatagtg aagccatgca acccagcctt
      721 agccgtagaa taattggtct gtccgaactg acctttctga ccattgaccg aactgatgtt
      781 tacgattctt
//
