LOCUS       sequence-115346-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tribolium basal heat shock promoter driving Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Gilles AF, Schinko JB, Averof M
  TITLE     Efficient CRISPR-mediated gene targeting and transgene replacement 
            in the beetle Tribolium castaneum.
  JOURNAL   Development. 2015 Jul 9. pii: dev.125054.
  PUBMED    26160901
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2015 Jul 9. pii: dev.125054."
COMMENT     Sequence Label: p(bhsp68-Cas9)
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             433..453
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(468..490)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      631..640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcgcctaggc cggccgaatt cgaatggcca tgggacgtcg acctgaggta attataaccc
       61 gggccctata tatggatcca attgcaatga tcatcatgac agatctgcgc gcgatcgata
      121 tccgtttcat atataagcgc ggtctcgcgg cgcgttgtca gtgaatttga atcaagcgaa
      181 gcgaaagcaa caaagctaag caagcgactc aaagcaaatt ttcaaagcga ctttaacaaa
      241 gttgagtgaa ttatttacta gtgaattcaa agtcgtcgat aaataagttt cccattttta
      301 ttccccagcc aggaagttag tttcaatagt tttgtaattt caacgaaact catttgattt
      361 cgtactaatt ttccacatct ctattttctg cccgcagaat aatccaaaat gcagatcggg
      421 gatcccaccc cacccaagaa gaagcgcaag gtggaggacg atcccgtcgt tttacaacgt
      481 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      541 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcggtcg
      601 actctagagg atccccggga tccaccggtc gccaccatgg ataagaaata ctcaataggc
      661 ttagatatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata taaggttccg
      721 tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa aaatcttata
      781 ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtctcaa acggacagct
      841 cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat tttttcaaat
      901 gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt t
//
