LOCUS       sequence-115347-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tribolium basal heat shock promoter driving Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Gilles AF, Schinko JB, Averof M
  TITLE     Efficient CRISPR-mediated gene targeting and transgene replacement 
            in the beetle Tribolium castaneum.
  JOURNAL   Development. 2015 Jul 9. pii: dev.125054.
  PUBMED    26160901
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2015 Jul 9. pii: dev.125054."
COMMENT     Sequence Label: p(bhsp68-Cas9)
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(429..449)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcgcgccaa gcttaaggtg cacggcccac gtggccacta gtgagcagcc agttgttttt
       61 ttctatattc ctgatgggca tacgaaggag agcccgtttg cgcttaatac tgccaaggcc
      121 acgactaatg cggtaagttt tttattattt taaaatgaaa caacttttta tctttaaatt
      181 aaataattta ttaaaaattt ttaaataatc tacaaaaatc agtctttaaa cttaatgcag
      241 tcactatcta ctttattata actaaagtaa ctttacgtac tattatttac aaaaataaac
      301 tatcacaatt aactaagtag gaacacaaac cgatcatctc gagctctgta catgtccgcg
      361 gtcgcgacgt acgcgtatcg atggcgccag ctgcaggcgg ccgctcatcc tgcagctcca
      421 ccgctcgaga ctttcctctt cttcttggga gaaccgtcac ctcctagctg actcaaatca
      481 atgcgtgttt cataaagacc agtgatggat tgatggataa gagtggcatc taaaacttct
      541 tttgtagacg tatatcgttt acgatcaatt gttgtatcaa aatatttaaa agcagcggga
      601 gctccaagat tcgtcaacgt aaataaatga ataatatttt ctgcttgttc acgtattggt
      661 ttgtctctat gtttgttata tgcactaaga actttatcta aattggcatc tgctaaaata
      721 acacgcttag aaaattcact gatttgctca ataatctcat ctaaataatg cttatgctgc
      781 tccacaaaca attgtttttg ttcgttatct tctggactac ccttcaactt ttcataatga
      841 ctagctaaat ataaaaaatt cacatatttg cttggcagag ccagctcatt tcctttttgt
      901 aattctccgg cactagccag catccgttta cgaccgtttt ctaactcaaa aagactatat
      961 ttaggtagtt taatgattaa gtctttttta acttccttat atcctttagc ttctaaaaag
     1021 tcaatcggat ttttttcaaa ggaacttctt tccataattg tgatccc
//
