LOCUS       sequence-115356-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for murine Fc-FLAG, used for monomeric 
            Fc-IL2 production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Zhu EF, Gai SA, Opel CF, Kwan BH, Surana R, Mihm MC, Kauke MJ, 
            Moynihan KD, Angelini A, Williams RT, Stephan MT, Kim JS, Yaffe MB, 
            Irvine DJ, Weiner LM, Dranoff G, Wittrup KD
  TITLE     Synergistic Innate and Adaptive Immune Response to Combination 
            Immunotherapy with Anti-Tumor Antigen Antibodies and Extended Serum 
            Half-Life IL-2.
  JOURNAL   Cancer Cell. 2015 Apr 13;27(4):489-501. doi: 
            10.1016/j.ccell.2015.03.004.
  PUBMED    25873172
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2015.03"; journalName: "Cancer Cell"; date: 
            "2015-04-13- 13"; volume: "27"; issue: "4"; pages: "489-501"
COMMENT     Sequence Label: FcD265A-FLAG
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..135)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accaacacac agatctctag agctctgatc ttttattagc cagaagtgat ctggatccag
       61 gcgcctggtc tagagcggcc gcgatatctg atcacacgtg tcgacttatc acttgtcatc
      121 gtcgtccttg tagtcagatc cgccaccttt acccagagac cgggagatgg tcttagtcgt
      181 aaggtgattg tgcagaccct cgtggaccac tgagcaggcg aaaagacttc ctctttccca
      241 agtgctcttt tgtactctga gcttgctgta catgaagtaa gaaccatcag agtccaggac
      301 tgttgcggtg ttcttgtagt tttgctctgt acgcccattg ctggtccagt ccacagcaat
      361 ttcggcaggt aagaagcctg tgatcatgca ggtcagactg aactctttct tagtcatctc
      421 ttctgctggt ggaggcaaga catatacctg tggagctctt actggccctc tgggttttga
      481 gatggttttc tcgatggggg atgggagggc tctgttgttg accttgcatt tgaactcctt
      541 gccactcatc cagtcctggt gctggatggg gagggcactg accacccgga gagtactgtt
      601 gtaatcctct ctatgggttt gtgtctgagc tgtgtgtact tccacgttgt tcacaaacca
      661 gctgatctgg acgtctgggt catcctcgct cacggccacc accacacatg tgaccatggg
      721 gctcagggag atcatgagta catccttgat ctttggagg
//
