LOCUS       sequence-115370-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PIq driving expression LacI-T, the Lactose inducible 
            chimera with the TAN DBD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[Iq:LacI-T]
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..74
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        139..216
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(236..255)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gccacctgac gtgctagcct aagaaaccat tattatcatg acattaacct ataaaaatag
       61 gcgtatcacg aggccctttc gtcttcacct cgacaattcg cgcgcgaagg cgaagcggca
      121 tgcatttacg ttggtaccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga
      181 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat
      241 gtcgcagagt atgccggtgt ctctacggcg accgtttcca acgtggtgaa ccaggccagc
      301 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
      361 cccaaccgcg tggcacaacn actggcgggc aaacagtcgt tgctgattgg cgttgccacc
      421 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
      481 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
      541 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
      601 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
      661 gatgtctctg ac
//
