LOCUS       sequence-115372-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PI driving expression FruR-L, and PI driving expression of 
            RbsR-L..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZM3[I:FruR-L,I:RbsR-L]
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt agctacggct gagcacgccg
       61 cggcgataga gattacgttt aatgcgcgtt aaaccaggtt ttggcttacg cggttcgtcc
      121 aggcttgcca ggacaatctc cagcacacgc tctgcgacat cgcggtgacg ttgagccact
      181 gccagcaccg gacactgtaa gaagtcgagc agttcgttat cgccaaaggt ggcaattgcc
      241 aggtcagaag gcagtttgcc gtcgcgacgc agcgtgacat ccatcactcc ttgcaacaac
      301 gcaaacgacg ttgtgaacag cgcctgcggc atcggatgcg tttccagcca tttttcgaat
      361 aactgggcag ccgcctcccg ctcatagctg ttggcataca ggaaatgcac ttcgcgcgga
      421 tcatctttcc aggcagtacg gaaaccttgt tcacgcagga agctgacaga aagctccggt
      481 agcgcaccaa gataaagcac cgtctcggcg ggaaacttac gtaactcttc cgccagcatt
      541 tcggcatcat cctgatcggc accaaccacg ctggtgaagt gttcacgatc gagggcgcgg
      601 tccagcgcga caatcgggaa cgggtcgtta gcccagcgtt gataaaaagg atgctcagga
      661 ggcaacgacg tcgaaacaat aatggcatca acctgacgct gtaaaaggtg ctcaatgcac
      721 cgcatttcgt tgtctggctg atcttctgag caggcaatca gcagttgata accccgttgc
      781 cgcgcctggc gttcaagata gttagcgatg cgggtatagc tggtgttctc cagatcgggg
      841 atcacaag
//
