LOCUS       sequence-115394-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor backbone vector to clone left and right HR arms in for 
            Degron-KI application.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Zhou Q, Derti A, Ruddy DA, Rakiec DP, Kao I, Lira M, Gibaja V, Chan 
            H, Yang Y, Min J, Schlabach MR, Stegmeier F
  TITLE     A chemical genetics approach for the functional assessment of novel 
            cancer genes.
  JOURNAL   Cancer Res. 2015 Mar 18. pii: canres.2930.2014.
  PUBMED    25788694
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2015 Mar 18. pii: canres.2930.2014."
COMMENT     Sequence Label: Degron-KI donor backbone
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..62)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(66..85)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(67..85)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(96..113)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(96..112)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(105..127)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        428..532
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catatgcgga ctgcttccgc tgagctcatg gcgcgcctag gccttgacgg ccttccttca
       61 attcgcccta tagtgagtcg tattacgtcg cgctcactgg ccgtcgtttt acaacgtcgt
      121 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      181 agctggcgta atagcgaaga ggcccgcacc gaaacgccct tcccaacagt tgcgcagcct
      241 gaatggcgaa tgggagcgcc ctgtagcggc cactcaaccc tatctcggtc tattcttttg
      301 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      361 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
      421 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      481 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgattga
      541 acaggatggc ctgcatgcgg gtagcccggc agcgtgggtg gaacgtctgt ttggctatga
      601 ttgggcgcag cagaccattg gctgctctga tgcggcggtg tttcgtctga gcgcgcaggg
      661 tcgtccggtg ctgtttgtga aaaccgatct gagcggtgcg ctgaacgagc tgcaggatga
      721 agcggcgcgt ctgagctggc tggccaccac cggtgttccg tgtgcggcgg tgctggatgt
      781 ggtgaccgaa gcgg
//
