LOCUS       sequence-115409-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor backbone vector to clone left and right HR arms in for 
            Degron-KI application.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Zhou Q, Derti A, Ruddy DA, Rakiec DP, Kao I, Lira M, Gibaja V, Chan 
            H, Yang Y, Min J, Schlabach MR, Stegmeier F
  TITLE     A chemical genetics approach for the functional assessment of novel 
            cancer genes.
  JOURNAL   Cancer Res. 2015 Mar 18. pii: canres.2930.2014.
  PUBMED    25788694
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2015 Mar 18. pii: canres.2930.2014."
COMMENT     Sequence Label: Degron-KI donor backbone
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(168..185)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(358..377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataagctcct gagcggccgc ggacgactag ctaaccgcgg tacctcttaa ttaactggcc
       61 tcatgggcct tcctttcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      121 taacatggtc atagctgttt ccttgcgtat tgggcgctct ccgcttcctc gctcactgac
      181 tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa
      241 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      301 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      361 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      421 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      481 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      541 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      601 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      661 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      721 cagtatt
//
