LOCUS       sequence-11546-e        8535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Williams KJ, Joyce G, Robertson BD
  TITLE     Improved mycobacterial tetracycline inducible vectors.
  JOURNAL   Plasmid. 2010 Sep;64(2):69-73. doi: 10.1016/j.plasmid.2010.04.003. 
            Epub 2010 Apr 29.
  PUBMED    20434484
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2010.04.003"; journalName: "Plasmid"; date: 
            "2010-09"; volume: "64"; issue: "2"; pages: "69-73"
COMMENT     Sequence Label: pKW08-Lx-Int
FEATURES             Location/Qualifiers
     source          1..8535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      14..41
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2903..2919)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2904..2920)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      3693..3739
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      7700..8288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8189..8208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8442..8459
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgattagcta agcagaaggc catcctgacg gatggccttt ttgcgtttaa tactgtttaa
       61 acctctagat cacgattcgc tcgaggtcaa tgacgctgtg cgggagtgag agaatccgtc
      121 gatcagtgct gtcaggccga aatcgaattc ccggtcgcgt tcaggaacgc tcgcattgcc
      181 gacctctgcg gtttgctcct ccagtacgac accgacggtg tatcggctga ccgcgatgag
      241 catgctgatg gctgtctgtt catcgaatcc ctgagacacg agaagcccga cttgctgacc
      301 ggaagcctca gctccttcgt cggtgtcgtc gccggatggt ctgcggtggt gctcagcgtg
      361 gagacgcgca ccgtcacgga ccgcgtgtag tgcgtggcgg aaacttcttg cgtttcgcaa
      421 gagaaatgcg tcccatttct cgtcggactc ggggaaggaa gcgtgatgct ctcggtcaag
      481 cacgtcgctc gccagcgctg cgaggagttc ggccttcgtg cggaagtgcc agtagaggcc
      541 gggctgctgt acctgtaagt gagccgccag cgcgcgagtg gtgaagccat cgagcccagt
      601 ctcgtcgagc acctgccggg ccccgagcaa cacggacgtg cggtcgagac gcttccggtg
      661 gtgagtcata gttgcacttt atcatcgata actttatctt agataaagtg actgctcgct
      721 actctcatct gactgctcgc tactctcatc gtggaatcct gacaggatcc ggaggaaatg
      781 ttatgaaatt tggaaacttc ctcctcactt atcagccacc tgagctatct cagaccgaag
      841 tgatgaagcg attggttaat ctgggcaaag cgtctgaagg ttgtggcttc gacaccgttt
      901 ggttgctaga gcaccacttc actgaatttg ggttgttagg gaatccttat gttgctgccg
      961 cacacctatt aggtgcgaca gaaacgctca acgttggcac tgcagctatc gtattgccga
     1021 ctgcccatcc ggttcgacaa gcagaagacg taaacctact ggatcaaatg tcaaaaggac
     1081 gattccgttt tggtatttgt cgcggtttgt acgataaaga ttttcgtgtc tttggtacag
     1141 acatggataa cagccgagcc ttaatggact gttggtatga cttgatgaaa gaaggcttca
     1201 atgaaggcta tatcgcggcg gataacgaac atattaagtt cccgaaaatc caactgaatc
     1261 catcggctta cacacaaggt ggtgctcctg tttatgtcgt cgcggagtca gcatcaacga
     1321 cagaatgggc tgcagagcgt ggcctaccaa tgattctaag ctggatcatc aacactcacg
     1381 agaagaaagc gcagcttgat ctttacaacg aagtcgcgac tgaacatggc tacgatgtga
     1441 ctaagattga ccactgtttg tcttacatca cctccgtcga tcatgactca aatagagcca
     1501 aagatatttg ccgcaacttc ttgggccatt ggtacgactc atacgtgaat gccaccaaga
     1561 tttttgacga ctctgaccaa acaaaaggtt acgacttcaa taaaggtcaa tggcgtgatt
     1621 ttgtgttgaa aggccacaaa gacaccaatc gccgaattga ttacagctac gaaatcaacc
     1681 cagtagggac gcctgaagag tgtatcgcga ttatccagca agatattgat gcgacgggta
     1741 ttgacaatat ttgttgtggt tttgaagcaa acggttctga agaagaaatt atcgcatcta
     1801 tgaagctatt ccagtctgat gtgatgccat atctcaaaga aaaacagtaa ttaatatttt
     1861 ctaaaaggaa agagacatga aatttggatt attcttcctc aattttatga actcaaagcg
     1921 ttcttctgat caagtcatcg aagaaatgtt agataccgca cattacgtag atcagttgaa
     1981 gtttgacacg ttggctgttt acgaaaacca tttctcgaac aatggtgtgg ttggtgcccc
     2041 actaacagtg gctggttttt tacttggtat gacaaagaac gccaaagtgg cttcgttgaa
     2101 tcacgtcatt accacgcatc atccagtacg tgtggcggaa gaagcgtgtc tacttgacca
     2161 aatgagtgaa ggccgttttg cctttggctt tagtgattgt gaaaagagtg cagatatgcg
     2221 cttctttaat cgaccaacgg attctcagtt tcagttgttc agtgagtgtc acaagatcat
     2281 caatgattca ttcactactg ggtactgcca tccaaacaat gatttttata gttttcctaa
     2341 aatctccgtt aacccacacg cgttcactga aggcggtcct gcgcaatttg tgaatgcgac
     2401 gagcaaagaa gtggttgaat gggcggctaa gttagggctt ccactcgtgt ttagatggga
     2461 cgactcaaac gctcaaagaa aagaatacgc cggtttgtac cacgaagttg ctcaggcaca
     2521 tggtgtcgat gttagtcagg ttcgacacaa gctgacgctg ctggtcaacc aaaatgtaga
     2581 tggtgaagca gcaagggcag aagctcgcgt gtatttggaa gagtttgtcc gtgaatctta
     2641 ctcaaatacc gactttgagc aaaaaatggg agagctgttg tcagaaaatg ccatcggtac
     2701 ttatgaagaa agtactcagg cagcgcgagt tgcgattgag tgttgtggtg ccgcggacct
     2761 attgatgtct tttgagtcga tggaagataa agcgcagcaa agagcggtta tcgatgtggt
     2821 aaacgccaac atcgtcaaat accactcgta aaagctttag ctaattaatt ggggacccta
     2881 gaggtcccct tttttatttt atcgataccg tcgacctcga gggggggccc gccggggatc
     2941 atcgagccga gaacgttatc gaagttggtc atgtgtaatc ccctcgtttg aactttggat
     3001 taagcgtaga tacacccttg gacaagccag ttggattcgg agacaagcaa attcagcctt
     3061 aaaaagggcg aggcctgcgg tggtggaaca ccgcagggcc tctaaccgct cgacgcgctg
     3121 caccaaccag cccgcgaacg gctggcagcc agcgtaaggc gcggctcatc gggcggcgtt
     3181 cgccacgatg tcctgcactt cgagccaagc ctcgaacacc tgctggtgtg cacgactcac
     3241 ccggttgttg acaccgcgcg cggccgtgcg ggctcggtgg ggcggctgtg tcgcccttgc
     3301 cagcgtgagt agcgcgtacc tcacctcgcc caacaggtcg cacacagccg attcgtacgc
     3361 cataaagcca ggtgagccca ccagctccgt aagttcgggc gctgtgtggc tcgtacccgc
     3421 gcattcaggc ggcagggggt ctaacgggtc taaggcggcg tgtacggccg ccacagcggc
     3481 tctcagcggc ccggaaacgt cctcgaaacg acgcatgtgt tcctcctggt tggtacaggt
     3541 ggttgggggt gctcggctgt cgctggtgtt ccaccaccag ggctcgacgg gagagcgggg
     3601 gagtgtgcag ttgtggggtg gcccctcagc gaaatatctg acttggagct cgtgtcggac
     3661 catacaccgg tgtacgatcg actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     3721 actgggcctt tcgttttatg ccatcatggc cgcggtgatc agctagagcc gtgaacgaca
     3781 gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct
     3841 tgtcccggtc tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg
     3901 ttcgcgcagg cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg
     3961 cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa
     4021 gaaaaatggc cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa
     4081 cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca catatatggg ccggtcaaga
     4141 taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa
     4201 gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt tcacgacgag
     4261 aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc agcgggagga
     4321 tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg
     4381 acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc gagatggaga
     4441 cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc acgctggagg
     4501 agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg gatctgtaca
     4561 gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc acagagatga
     4621 cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg actgcccgcc
     4681 ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac aagctgatcg
     4741 cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta gaggcgctga
     4801 cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg atcgcggcat
     4861 acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc cgcaaggaca
     4921 tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga
     4981 acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc
     5041 ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg aacaagggcc
     5101 ccgaggcatt cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca
     5161 agtcgctgaa gcgtggctac gccaagatcg gtcggccgga actccgcatc cacgacctcc
     5221 gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac gaccaaggag ctgatggccc
     5281 gtctcggtca cacgactcct aggatggcga tgaagtacca gatggcgtct gaggcccgcg
     5341 acgaggctat cgctgaggcg atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc
     5401 cccctcccaa ggacactgag tcctaaagag gggggtttct tgtcagtacg cgaagaacca
     5461 cgcctggccg cgagcgccag caccgccgct ctgtgcggag acctgggcac cagccccgcc
     5521 gccgccagga gcattgccgt tcccgccagc tagcaacaat tggggagcgg gtgtcgcggg
     5581 ggttccgtgg ggggttccgt tgcaacgggt cggacaggta aaagtcctgg tagacgctag
     5641 ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg ttgcgcccgt tttgaatacc
     5701 agccagacga gacggggttc tacgaatctt ggtcgatacc aagccatttc cgctgaatat
     5761 cggggagctc accgccagaa tcggtggttg tggtgatgta cgtggcgaac tccgttgtag
     5821 tgcctgtggt ggcatccgtg gccactctcg ttgcacggtt cgttgtgccg ttacaggccc
     5881 cgttgacagc tcaccgaacg tagttaaaac atgctggtca aactaggttt accaacgata
     5941 cgagtcagct catctagggc cagttctagg cgttgttcgt tgcgcggttc gttgcgcatg
     6001 tttcgtgtgg ttgctagatg gctccgcaac cacacgcttc gaggttgagt gcttccagca
     6061 cgggcgcgat ccagaagaac ttcgtcgtgc gactgtcctc gttgatcctt gccgagctgg
     6121 gatggaagct cggccgacca ccctggagga gatgatcgag gatgccaggg cctttcacgc
     6181 ccgccgctgc tgagcgtccg ccgccgggcc cgcaccgccg tcggccggcc cgctccgggc
     6241 tcgcagcagc gggcttcggc gcgggcccgg ggctcccgag cgcgggcggg gctccgggcg
     6301 gccgccgggg gccgggggcg gcgccgggcg gcccggggcg tcaggcgccg ggggcggtgt
     6361 ccggcggccc ccagaggaac tgcgccagtt cctccggatc ggtgaagccg gagagatcca
     6421 gcggggtctc ctcgaacacc tcgaagtcgt gcaggaaggt gaaggcgagc agttcgcggg
     6481 cgaagtcctc ggtccgcttc cactgcgccc cgtcgagcag cgcggccagg atctcgcggt
     6541 cgccccggaa ggcgttgaga tgcagttgca ccaggctgta gcgggagtct cccgcataga
     6601 cgtcggtgaa gtcgacgatc ccggtgacct cggtcgcggc caggtccacg aagatgttgg
     6661 tcccgtgcag gtcgccgtgg acgaaccggg gttcgcggcc ggccagcagc gtgtccacgt
     6721 ccggcagcca gtcctccagg cggtccagca gccggggcga gaggtagccc cacccgcggt
     6781 ggtcctcgac ggtcgccgcg cggcgttccc gcagcagttc cgggaagacc tcggaatggg
     6841 gggtgagcac ggtgttcccg gtcagcggca ccctgtgcag ccggccgagc acccggccga
     6901 gttcgcgggc cagggcgagc agcgcgttcc ggtcggtcgt gccgtccatc gcggaccgcc
     6961 aggtggtgcc ggtcatccgg ctcatcacca ggtagggcca cggccaggct ccggtgccgg
     7021 gccgcagctc gccgcggccg aggaggcggg gcaccggcac cggggcgtcc gccaggaccg
     7081 cgtacgcctc cgactccgac gcgaggctct ccggaccgca ccagtgctcg ccgaacagct
     7141 tgatcaccgg gtcgggctcg ccgaccagta cggggttggt gctctcgccg ggcacccgca
     7201 gcaccggcgg caccggcagc ccgagctcct ccagggctcg gcgggccagc ggctcccaga
     7261 attcctggtc gttccgcagg ctcgcgtagg aatcatccga atcaatacgg tcgagaagta
     7321 acagggattc ttgtgtcaca gcggacctct attcacaggg tacgggccgg cttaattccg
     7381 cacggccggt cgcgacacgg cctgtccgca ccgcggtcag gcgttgacga tgacgggctg
     7441 gtcggccacg tccggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7501 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7561 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7621 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7681 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7741 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7801 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7861 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7921 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7981 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8041 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8101 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8161 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8221 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8281 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8341 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8401 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8461 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8521 gcagctagaa ctagt
//
