LOCUS       sequence-115460-        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP56-7
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..32
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(70..92)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(364..439)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(460..809)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     815..837
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     829..846
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     830..846
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    854..874
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
       61 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
      121 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
      181 gcttaactat gcggcatcag agcagattgc actgagagtg caccatatgc ggtgtgaaat
      241 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
      301 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccaga aatttcacaa
      361 aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg
      421 ctatttctag ctctaaaacg tagtcacggc gatgattaca agacatctcg caataggagg
      481 tgtttatata ggggcaacac aagaggcggc agacatctcg tgagtttgac agagtagtat
      541 aaagagtgtg tgtgagaggg tgtaggcatt tcataacata ttttttgtgg aataattttg
      601 gaaaaaaggt gtttttttaa taatagcttt tataagagtt tctcaaaaat tgcatgcttt
      661 ttttgaaaag gttgacaaat tatagtagta ctaaagttgc tattttcagg tctcagcaga
      721 aaattcacaa ctaaaaataa aatttgcaaa acagagtgtg cgcctttaag gtgtacagtt
      781 ttttattcct ttattgctag ttttttttgg ttttcccagt cacgacgttg taaaacgacg
      841 gccagtgaat tatcaactat gtataataaa gttgtgagac ttttttcttg gcggcacaat
      901 aaagtttcct ctttttgttg ggtcactcaa cgaacacaat acaaatattt gagcgctcgc
      961 atttttctca acctttcggg tttaaagcac ttcaaaatgg aattgaagag agattgtgaa
     1021 agtacatcag acagaatcac attttgatag cattcacttc actcagatgc aaacttttca
     1081 gtgactataa aaagtc
//
