LOCUS       sequence-115462-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 4-6 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX2-44
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(388..405)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(388..404)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(397..419)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      468..554
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      646..673
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgtcacccg gcaaccttgg gtagcagcga agtcgaggca tttctgtcct ggctggtcta
       61 gacgtctcgc catggtcctg gcacagcacc ggcaacagcc gctgcaccgt ttcgagcgct
      121 tgcttgccgc caccgttgct ggcgatagcc accacttggt ccggggtcag gccatggtcc
      181 tggcacagca ccggcaacag ccgctgcacc gtttcgagcg cttgcttgcc gccattgttg
      241 ctggcgatag ccaccacttg gtccggggtc aggccatggt cctggcacag caccggcaac
      301 agccgctgca ccgtttcgag cgcttgcttg ccgccattgt tgctggcgat agccaccact
      361 tggtccgggg tcagggagac gcttaagact ggccgtcgtt ttacaacgtc gtgactggga
      421 aaacatccat gctagcgtta acgcgagagt agggaactgc caggcatcaa ataaaacgaa
      481 aggctcagtc ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      541 tgagtaggac aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg cccggagggt
      601 ggcgggcagg acgcccgcca taaactgcca ggcatcaaac taagcagaag gccatcctga
      661 cggatggcct ttttgcgttt cta
//
