LOCUS       sequence-115465-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP56-8
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..22
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(26..349)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(364..390)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(391..411)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcaactatgt ataataaagt tgtgagactt ttttcttggc ggcacaataa agtttcctct
       61 ttttgttggg tcactcaacg aacacaatac aaatatttga gcgctcgcat ttttctcaac
      121 ctttcgggtt taaagcactt caaaatggaa ttgaagagag attgtgaaag tacatcagac
      181 agaatcacat tttgatagca ttcacttcac tcagatgcaa acttttcagt gactataaaa
      241 agtcataaaa taataacatt aaaaaagcaa ataaattagc gagagaattt tttgacaaaa
      301 agaaagaaga gtgatagaga agaagggaat gcttgaaagg attttgcatt tatcactagt
      361 ttaggcgtag tctgggacgt cgtatgggta gaccttacgc ttcttctttg gtggtccgga
      421 tcctccgtct cctccgagtt gggagaggtc gatacgggtc tcgtagagtc cggtgatgga
      481 ttggtggatg agggtggcgt cgaggacctc cttggtggag gtgtaacgct tacggtcgat
      541 ggtggtgtcg aagtacttga aggcggctgg ggctccgagg ttggtgaggg tgaagaggtg
      601 gatgatgttc tcggcttgct cacggattgg cttgtcacgg tgcttgttgt aggcggagag
      661 gaccttgtcg aggttggcgt cggcgaggat gacacgcttg gagaactcgg agatttgctc
      721 gatgatctcg tcgaggtagt gcttgtgttg ctcgacgaag agttgctttt gctcgttgtc
      781 ctctggggat cccttgagct tctcgtagtg ggaggcgagg tagaggaagt tgacgtactt
      841 ggatgggagg gcgagctcgt ttcccttttg gagctctccg gcggaggcga gcatacgctt
      901 acgtccgttc tcgagctcga agagggagta ctttgggagc ttgatgatga ggtccttctt
      961 gacctccttg tatcccttgg cctcgaggaa gtc
//
