LOCUS       sequence-115470-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 7-9 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX3-12
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(402..419)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(402..418)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(411..433)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      482..568
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      660..687
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(846..863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagacgt ctcgcaggcc atggtcctgg cacagcaccg gcaacagccg ctgcaccgtt
      121 tcgagcgctt gcttgccgcc accgttgctg gcgatagcca ccacttggtc cggggtcagg
      181 ccatggtcct ggcacagcac cggcaacagc cgctgcaccg tttcgagcgc ttgcttgccg
      241 ccattgttgc tggcgatagc cacgacctga tcgggtgtga ggccatggtc ctggcacagc
      301 accggcaaca gccgctgcac cgtttcgagc gcttgcttgc cgccaatgtt gctggcgata
      361 gccaccactt ggtccggggt caggccatgg agacgcttaa gactggccgt cgttttacaa
      421 cgtcgtgact gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca
      481 tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc
      541 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca
      601 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca
      661 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta
      721 cgcgtattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      961 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgc
//
